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Image Search Results


Cre-dependent ClopHensorN for targeting expression to genetically defined neuronal subtypes

Journal: iScience

Article Title: A genetically targeted ion sensor reveals distinct seizure-related chloride and pH dynamics in GABAergic interneuron populations

doi: 10.1016/j.isci.2023.106363

Figure Lengend Snippet: Cre-dependent ClopHensorN for targeting expression to genetically defined neuronal subtypes

Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Bacterial and Virus Strains AAV8-EF1a-double floxed-ClopHensorN-WPRE-HGHpA The Vector Core at the University of North Carolina at Chapel Hill ( UNC Vector Core ) AAV custom production using this paper’s floxed ClopHensorN plasmid made available on Addgene Cat#193728 Chemicals, Peptides, and Recombinant Proteins Earle’s Balanced Salt Solution with CaCl 2 and MgSO 4 Thermo Fisher Scientific Cat#24010043 Millicell-CM membranes Sigma-Aldrich (USA) Cat#PICM03050 Minimum Essential Media with GlutaMAX-I Thermo Fisher Scientific Cat#41090036 Heat-inactivated horse serum Thermo Fisher Scientific Cat#26050088 B27 supplement Thermo Fisher Scientific Cat#17504044 Nigericin Sigma-Aldrich (USA) Cat#N7143 Tributyltin chloride Sigma-Aldrich (USA) Cat# T50202 Chloride ionophore I Sigma-Aldrich (USA) Cat#99408-0.1 ML-F Experimental Models: Cell Lines HEK293 cells Sigma-Aldrich (USA) Cat#85120602 Experimental Models: Organisms/Strains Mouse: PV-Cre: B6; 129P2-Pvalb tm1(cre)Arbr /J The Jackson Laboratory (USA) RRID:IMSR_JAX:008069 Mouse: SST-IRES-Cre: Sst tm2.1(cre)Zjh /J The Jackson Laboratory (USA) RRID:IMSR_JAX:013044 Mouse: VIP-IRES-Cre: Vip tm1(cre)Zjh /J The Jackson Laboratory (USA) RRID:IMSR_JAX:010908 Mouse: CamK2a-Cre: Tg(Camk2a-cre)T29-1Stl/J The Jackson Laboratory (USA) RRID:IMSR_JAX:005359 Mouse: C57BL/6J: C57BL/6J The Jackson Laboratory (USA) RRID:IMSR_JAX:000664 Recombinant DNA ClopHensorN plasmid Addgene Cat#50758 floxed ClopHensorN plasmid Addgene Cat#193728 Software and Algorithms MATLAB Mathworks (USA) R2017b GraphPad Prism GraphPad Software (USA) v6.01 CorelDraw Corel Corporation (USA) X6 Open in a separate window Key resources table .

Techniques: Expressing

ClopHensorN calibration

Journal: iScience

Article Title: A genetically targeted ion sensor reveals distinct seizure-related chloride and pH dynamics in GABAergic interneuron populations

doi: 10.1016/j.isci.2023.106363

Figure Lengend Snippet: ClopHensorN calibration

Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Bacterial and Virus Strains AAV8-EF1a-double floxed-ClopHensorN-WPRE-HGHpA The Vector Core at the University of North Carolina at Chapel Hill ( UNC Vector Core ) AAV custom production using this paper’s floxed ClopHensorN plasmid made available on Addgene Cat#193728 Chemicals, Peptides, and Recombinant Proteins Earle’s Balanced Salt Solution with CaCl 2 and MgSO 4 Thermo Fisher Scientific Cat#24010043 Millicell-CM membranes Sigma-Aldrich (USA) Cat#PICM03050 Minimum Essential Media with GlutaMAX-I Thermo Fisher Scientific Cat#41090036 Heat-inactivated horse serum Thermo Fisher Scientific Cat#26050088 B27 supplement Thermo Fisher Scientific Cat#17504044 Nigericin Sigma-Aldrich (USA) Cat#N7143 Tributyltin chloride Sigma-Aldrich (USA) Cat# T50202 Chloride ionophore I Sigma-Aldrich (USA) Cat#99408-0.1 ML-F Experimental Models: Cell Lines HEK293 cells Sigma-Aldrich (USA) Cat#85120602 Experimental Models: Organisms/Strains Mouse: PV-Cre: B6; 129P2-Pvalb tm1(cre)Arbr /J The Jackson Laboratory (USA) RRID:IMSR_JAX:008069 Mouse: SST-IRES-Cre: Sst tm2.1(cre)Zjh /J The Jackson Laboratory (USA) RRID:IMSR_JAX:013044 Mouse: VIP-IRES-Cre: Vip tm1(cre)Zjh /J The Jackson Laboratory (USA) RRID:IMSR_JAX:010908 Mouse: CamK2a-Cre: Tg(Camk2a-cre)T29-1Stl/J The Jackson Laboratory (USA) RRID:IMSR_JAX:005359 Mouse: C57BL/6J: C57BL/6J The Jackson Laboratory (USA) RRID:IMSR_JAX:000664 Recombinant DNA ClopHensorN plasmid Addgene Cat#50758 floxed ClopHensorN plasmid Addgene Cat#193728 Software and Algorithms MATLAB Mathworks (USA) R2017b GraphPad Prism GraphPad Software (USA) v6.01 CorelDraw Corel Corporation (USA) X6 Open in a separate window Key resources table .

Techniques:

Key resources table

Journal: iScience

Article Title: A genetically targeted ion sensor reveals distinct seizure-related chloride and pH dynamics in GABAergic interneuron populations

doi: 10.1016/j.isci.2023.106363

Figure Lengend Snippet: Key resources table

Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Bacterial and Virus Strains AAV8-EF1a-double floxed-ClopHensorN-WPRE-HGHpA The Vector Core at the University of North Carolina at Chapel Hill ( UNC Vector Core ) AAV custom production using this paper’s floxed ClopHensorN plasmid made available on Addgene Cat#193728 Chemicals, Peptides, and Recombinant Proteins Earle’s Balanced Salt Solution with CaCl 2 and MgSO 4 Thermo Fisher Scientific Cat#24010043 Millicell-CM membranes Sigma-Aldrich (USA) Cat#PICM03050 Minimum Essential Media with GlutaMAX-I Thermo Fisher Scientific Cat#41090036 Heat-inactivated horse serum Thermo Fisher Scientific Cat#26050088 B27 supplement Thermo Fisher Scientific Cat#17504044 Nigericin Sigma-Aldrich (USA) Cat#N7143 Tributyltin chloride Sigma-Aldrich (USA) Cat# T50202 Chloride ionophore I Sigma-Aldrich (USA) Cat#99408-0.1 ML-F Experimental Models: Cell Lines HEK293 cells Sigma-Aldrich (USA) Cat#85120602 Experimental Models: Organisms/Strains Mouse: PV-Cre: B6; 129P2-Pvalb tm1(cre)Arbr /J The Jackson Laboratory (USA) RRID:IMSR_JAX:008069 Mouse: SST-IRES-Cre: Sst tm2.1(cre)Zjh /J The Jackson Laboratory (USA) RRID:IMSR_JAX:013044 Mouse: VIP-IRES-Cre: Vip tm1(cre)Zjh /J The Jackson Laboratory (USA) RRID:IMSR_JAX:010908 Mouse: CamK2a-Cre: Tg(Camk2a-cre)T29-1Stl/J The Jackson Laboratory (USA) RRID:IMSR_JAX:005359 Mouse: C57BL/6J: C57BL/6J The Jackson Laboratory (USA) RRID:IMSR_JAX:000664 Recombinant DNA ClopHensorN plasmid Addgene Cat#50758 floxed ClopHensorN plasmid Addgene Cat#193728 Software and Algorithms MATLAB Mathworks (USA) R2017b GraphPad Prism GraphPad Software (USA) v6.01 CorelDraw Corel Corporation (USA) X6 Open in a separate window Key resources table .

Techniques: Virus, Plasmid Preparation, Recombinant, Software